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Are You Know What Is Exactly Chromatography?
At its beginning, chromatography was used to separate plant pigments into their contributing chemicals. A pigment, such as chlorophyll, would be marked onto a sheet of silica, glass, or plastic and soaked in an appropriate solvent. As the solvent moved across the sheet, then it dissolved the chlorophyll and smudged the initial mark. This smudge would slowly separate into various color bands as it travelled with the solvent. In this case, chlorophyll is the analyze mixture or sample, the sheet is the static phase, and the solvent is the mobile phase. Nowadays, most applications of chromatography are generally less vibrant but the underlying principle of separation remains unchanged. An assortment…